CytoSinct™ TCR αβ Nanobeads, human

The CytoSinct TCR αβ Nanobeads, human is used for in vitro depletion of TCRα/β+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products or single cell suspension based on the surface expression of human TCRα/β.

Price	$350.00
Cat. No.	L00897-1
Size	1 ml 7.5 ml : Quote Buy in bulk
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Overview Properties Bottom Notes

Overview

Product Description

The CytoSinct TCR αβ Nanobeads, human is used for in vitro depletion of TCR αβ⁺ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspension based on the surface expression of human TCR αβ.
To begin the isolation, Nanobeads are added to the cells. The cells/beads mixed suspension is loaded onto a CytoSinct column placed in the CytoSinct magnet, or other compatible columns with magnets.
The TCR αβ⁺ cells labeled with Nanobeads are retained within the column and unlabeled cells (TCR αβ^- cells) are flushed out during the wash step. After removing the column from the magnetic field, the retained TCR αβ⁺ cells can be eluted as the positively selected cell fraction.

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Properties

Capacity

Cat. No.	Product Name	Size	Capacity
L00897-1	CytoSinct™ TCR αβ Nanobeads, human	1 mL	Up to 1×10⁹ total cells
L00897-7.5	CytoSinct™ TCR αβ Nanobeads, human	7.5 mL	Up to 7.5×10⁹ total cells

Format

Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188.

Components

1 mL or 7.5 mL CytoSinct TCR αβ Nanobeads, human
Nanobeads conjugated to monoclonal anti-human TCR αβ antibodies.

Storage & Stability

Store at 2-8 °C. Do not freeze.

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TCR αβ⁺ T cells were depleted from human PBMCs. TCR αβ⁺ cells were stained with anti-TCR αβ (clone: BW242/412) PE and gated on Live/hCD45⁺. »

For research use only. Not intended for human and animal therapeutic or diagnostic use.