CytoSinct™ CD4 Nanobeads, human

CytoSinct CD4 Nanobeads, human is used for in vitro enrichment of CD4+ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspension based on the surface expression of human CD4.

Price	$180.00
Cat. No.	L00863-0.5
Size	500 μl 1 ml Buy in bulk
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Overview Properties Bottom Notes

Overview

Product Description

CytoSinct CD4 Nanobeads, human is used for in vitro enrichment of CD4⁺ cells from fresh or frozen peripheral blood mononuclear cells (PBMCs), leukapheresis products, or single-cell suspension based on the surface expression of human CD4.
To begin the isolation, Nanobeads are added to the cells. The cells/beads mixed suspension is loaded onto a CytoSinct column placed in the CytoSinct magnet, or other compatible columns with a magnet.
The CD4⁺ cells labeled with Nanobeads are retained within the column and unlabelled cells (CD4^- cells) are flushed out during the wash step. After removing the column from the magnetic field, the retained CD4⁺ cells can be eluted as the positively selected cell fraction.

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Properties

Format

Supplied in phosphate-buffered saline (PBS), containing Human Serum Albumin (HSA) and Poloxamer 188.

Components

1 mL or 0.5 mL CytoSinct CD4 Nanobeads, human
Nanobeads conjugated to monoclonal anti-human CD4 antibodies (isotype: mouse IgG1).

Volume

Cat. No.	Size	Capacity
L00863-0.5	500 µL	Up to 5 × 10⁸ total cells
L00863-1	1 mL	Up to 1 × 10⁹ total cells

Storage & Stability

Store at 2 - 8 °C. Do not freeze.

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CD4⁺ T cells were enriched from human PBMCs. CD4⁺ cells were stained with anti-CD4 (clone: SK3) APC and gated on Live/hCD45⁺. »

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.